SDS Buffer, 10% Sodium Dodecyl Sulfate Solution for Lab, 500mL2
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UYU 1968
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Sodium dodecyl sulfate (SDS) reagent with consistent quality, ideal for precise biochemical applications.
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What Stands Out
Detalles de producto
| Brand Name | CryoKing |
| Item Highlight | 10% SDS solution for SDS-PAGE, protein electrophoresis, plasmid extraction and molecular biology workflows |
| Unit Count | 2 Count |
| Manufacturer | BIOLOGIX |
| Included Components | 10% SDS Buffer |
| Part Number | SDS-500-2 |
| Brand | CryoKing |
| Item Highlight | 10% SDS solution for SDS-PAGE, protein electrophoresis, plasmid extraction and molecular biology workflows See more |
| Item Weight | 1.1 lbs (500 grams) |
What's in the box
10% SDS Buffer
DESCRIPCIÓN DEL PRODUCTO
Preguntas y respuestas de los clientes
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Pregunta:
What is Lab SDS Buffer and what are its applications?
Respuesta: Lab SDS Buffer, or Sodium Dodecyl Sulfate Buffer, is a commonly used detergent in biochemistry, especially in the preparation of samples for SDS-PAGE. It works by denaturing proteins, which allows for their separation based on size during electrophoresis. This buffer is essential in molecular biology for analyzing protein expression, purity, and molecular weight across various experiments. -
Pregunta:
How should I store Lab SDS Buffer?
Respuesta: Lab SDS Buffer should be stored at room temperature, away from direct sunlight and moisture. For extended storage, keeping it in a cool place is advisable. Proper storage prevents degradation and ensures that the buffer maintains its effectiveness for long-term use in various lab applications, such as gel electrophoresis and western blotting. -
Pregunta:
What is the concentration of this Lab SDS Buffer?
Respuesta: This Lab SDS Buffer has a concentration of 10% Sodium Dodecyl Sulfate. This concentration is optimal for creating a strong negative charge on proteins, facilitating their separation in gel electrophoresis. Various experiments leverage this concentration for reliable and reproducible results, particularly in protein analysis and interactions. -
Pregunta:
Can Lab SDS Buffer be used for both protein and nucleic acid analysis?
Respuesta: While Lab SDS Buffer is primarily designed for protein analysis, it can inadvertently affect nucleic acids. SDS can denature proteins, making them ideal for separating polypeptides in experiments, but it is not the best choice for nucleic acid extraction or analysis due to its nature of disrupting the secondary structures of DNA and RNA. -
Pregunta:
Is Lab SDS Buffer safe to handle?
Respuesta: Lab SDS Buffer is a chemical reagent and should be handled with care. It can cause skin and eye irritation, so using gloves, goggles, and working in a well-ventilated area is recommended. Being informed about safety procedures helps prevent accidents in the laboratory, ensuring a safer working environment. -
Pregunta:
What is the pH of Lab SDS Buffer?
Respuesta: The pH of Lab SDS Buffer is typically around 7.5, making it suitable for various biochemical applications, including protein solubilization and electrophoresis. Monitoring pH is crucial as it can significantly affect protein structure and function, ensuring accurate results in experimental setups. -
Pregunta:
How do I prepare Lab SDS Buffer if I need a different concentration?
Respuesta: To prepare Lab SDS Buffer at a different concentration, adjust the amount of Sodium Dodecyl Sulfate (SDS) and diluent accordingly. For example, to prepare a 1% SDS solution, mix 1 part SDS with 99 parts of buffer solution. Understanding these calculations allows for customization based on the needs of various experiments. -
Pregunta:
Can Lab SDS Buffer be used for protein purification?
Respuesta: Yes, Lab SDS Buffer can be used during the protein purification process. It aids in solubilizing membrane proteins and helps maintain denatured states for further separation. This buffer is often utilized in protocols where proteins need to be purified from complex mixtures, facilitating better visibility and analysis. -
Pregunta:
What should I do if my Lab SDS Buffer appears cloudy?
Respuesta: If your Lab SDS Buffer appears cloudy, this could indicate contamination or precipitation. It is essential to prepare a fresh batch for your experiments to ensure reliability and consistency in your results. Cloudy solutions can impact the performance of electrophoresis and compromise experimental outcomes. -
Pregunta:
Where can I buy Lab SDS Buffer, Lab 10% Sodium Dodecyl Sulfate Buffers, 500ml/Bottle in Uruguay?
Respuesta: You can buy Lab SDS Buffer, Lab 10% Sodium Dodecyl Sulfate Buffers, 500ml/Bottle at Ubuy. Ubuy offers a convenient online platform where you can browse through various laboratory supplies and make a purchase, ensuring that you get the quality products needed for your research.
CryoKing Buffers Editorial Review
SDS Buffer, 10% Sodium Dodecyl Sulfate Solution for Lab, 500mL2 is an essential tool for various molecular biology workflows, including SDS-PAGE, protein electrophoresis, and plasmid extraction. Manufactured by BIOLOGIX under the CryoKing brand, this 10% SDS solution is reliable for achieving optimal results in labs. With a weight of 1.1 lbs for the 500 grams of solution, it is designed for easy handling and use in research settings. Users have praised its efficiency in protein separation during gel electrophoresis, making it a staple in many laboratory setups.
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ventajas
- High-quality for protein electrophoresis
- Effective in plasmid extraction
- Reliable for SDS-PAGE applications
- Easy to handle with 500mL volume
- Trusted brand in molecular biology
Contras
- Requires careful handling and storage conditions
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características y beneficios
- Advanced technology for reliable biochemical applications.
- Long shelf life of at least 3 years when stored at room temperature.
- Low endotoxin levels achieved through one-step injection molding.
- Batch consistency rigorously tested for quality assurance.
- Suitable for a wide range of biochemical uses.
- Promotes precision and reliability in laboratory settings.
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